Radwa Taher Mostafa Mohamed Elsharkawy

Hesham S. Abdelmoneim

01/01/2020

Radwa Taher Mostafa Mohamed Elsharkawy

Nesreen Khairy; Rehab Tarek Elsharkawy

01/07/2019

Radwa Taher Mostafa Mohamed Elsharkawy

01/02/2019

Background: The tongue is the second most commonly affected oral site after periodontal tissues in diabetes. Diabetic complications are mainly attributed to oxidative stress and side effects from long term use of drugs to treat diabetes. MO has been reported to be a valuable source of natural antioxidants. Materials and methods: this study comprised three groups; control, diabetic and MO treated groups. The experiment was terminated after fourteen days. Evaluation of the rats was done by measuring blood glucose levels, as well as, histological assessment of the tongue tissue by H&E and SEM. Results: There was a significant reduction in blood glucose levels and histological evaluation revealed improvement in the condition of the tongue mucosa in the MO treated group compared to the diabetic group. Conclusion: MO has a hypoglycemic effect on the blood glucose level of diabetic albino rats. MO may partially reverse some of the detrimental effects hyperglycemia has on the tongue mucosa of diabetic rats.

Radwa Taher Mostafa Mohamed Elsharkawy

01/04/2018

Moringa Oleifera (MO), also known as the ‘drumstick tree,’ is recognized as a nutritious and cheap source of phytochemicals, that a have a prominent anti-oxidative effect. Salivary gland dysfunction has been frequently reported in diabetic patients. Diabetes is a chronic metabolic disease that has complications mainly resulting from persistent hyperglycemia. Aim of the study: To Assess the effect of MO aqueous leaf extract on blood glucose levels in diabetic albino rats and its effect on submandibular salivary glands of diabetic albino rats. Materials and methods: the study comprised three groups; control, diabetic and MO treated groups. The experiment was terminated after fourteen days. The evaluation was performed by measuring the blood glucose levels and weight. Histological evaluation was done by H&E, PAS and IHC for COX-2. Results: the blood glucose levels and histological signs of diabetic complications were significantly lowered in the MO treated group. Conclusion: MO is a promising anti-diabetic treatment and may even reverse some of the diabetic complications.

Radwa Taher Mostafa Mohamed Elsharkawy

Medhat A. El-Zainy, Ahmed Mahmoud Halawa

01/12/2017

The healing potential of bone is influenced by a variety of biochemical, cellular, hormonal and pathological mechanisms. As previous studies stated that parotid salivary glands may have endocrinal role, the aim of this study was to evaluate the effect of parotidectomy on bone healing and on calcium and amylase enzyme levels in blood. The rats were divided into two groups; control and experimental group. The control group was subjected to unilateral surgical mandibular defects, while the experimental group was subjected to the same procedure in addition to bilateral surgical removal of the parotid glands. Each of the control and experimental groups was further subdivided into 3 subgroups, A, B and C according to the time of termination corresponding to 4, 8 and 12 weeks respectively. Blood samples were obtained in order to determine calcium and amylase levels in blood. The surgically defected mandibles of each subgroup were analyzed postoperative to determine the radiographic bone density of the surgical defect throughout the healing process, then processed and examined histologically. Examination of the H & E stained sections of the mandibles at 4 weeks showed minimal bone formation from the defect margin of the experimental group in comparison with the control group. At 8 weeks, the experimental group showed increase of bone formation from the defect margin. At 12 weeks, the center of the defect was filled by a considerable amount of spongy bone and a definite reversal line between new and old bone. The Masson trichrome stained sections of the experimental group at 12 weeks presented a considerable amount of green collagen fibers. The average (mean) percentage of radiographic bone densities of the surgical defect of the experimental group slightly raised to 82.06 at 12 weeks. The serum amylase level at 4 weeks was less than the normal value then was slightly increased at 8 weeks and finally at 12 weeks increased more than the normal value. However, the serum calcium level was within the normal value in all experimental and control subgroups. It was concluded that bilateral parotidectomy in albino rats resulted in delayed bone healing and was associated with an initial drop in serum amylase level at 4 weeks, however serum amylase level was self-compensated at 8 and 12 weeks postoperatively, while it didn't significantly influence serum calcium level.

Radwa Taher Mostafa Mohamed Elsharkawy

Samia M.Kamal

01/01/2012

This study investigated the effect of Nigella Sativa (N.sativa) on the histological features of parotid salivary glands of aging albino rats and its role on the activity of the glands for secretion of insulin- and glucagonlike peptides. Forty five male albino rats aged ten months were kept on the laboratory diet over a period of two months. Then, they were divided into three equal groups; young control (sacrificed at the end of the two months), old control (sacrificed three months later) and the experimental group (supplemented with N. sativa in a daily dose of 300mg/200gm body weight over a period of three months and then sacrificed). The parotid glands were then dissected out and subjected to histological and immunohistochemical investigations. The results showed only minimal amount of fibrosis and inflammatory cell infiltration in the N. sativa supplemented group. There were no distinctive changes in the architecture of the glands compared to that of young control. They did not show the prominent extensive features of aging manifested in the old aged control group. Moreover, the N. sativa supplemented group showed obvious increase in immunohistochemical reactivities for insulin and glucagon in the glandular tissue when compared to the rats of old control. Finally it could be concluded that N.sativa has got a cytoprotective effect against the degenerative changes of age and a beneficial role on the integrity of parotid salivary glands of aged rat. Also, N. sativa has been shown to increase the activity of parenchymal cells of rat parotid gland for insulin and glucagon that was markedly diminished with advance of age